Part:BBa_K1039003

Bxb1 integrase

Bxb1 integrase is a serine integrase that catalyzes site-specific recombination between Bxb1 attP (BBa_K1039031) and attB (BBa_K1039032) sites to produce Bxb1 attR (BBa_K1039033) and attL (BBa_K1039034) sites. This recombination is unidirectional, i.e. attR and attL do not recombine in the presence of Bxb1 integrase alone. (Ghaosh et al., 2005).

In concert with Bxb1 recombination directionality factor (RDF) (BBa_K1039006), Bxb1 integrase catalyzes site-specific recombination between Bxb1 attR and attL sites, to produce Bxb1 attP and attB sites. This recombination is also unidirectional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of Bxb1 integrase and RDF.

Bxb1 integrase can be used to flip the Bxb1 invertible promoter switches BBa_K1039001 and BBa_K1039001 from PB state to RL state.

In concert with Bxb1 RDF (BBa_K1039006), Bxb1 integrase can be used to flip the Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039005 from RL state to PB state.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 192
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 466
  Illegal XhoI site found at 553
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1105
  Illegal NgoMIV site found at 1192
  Illegal AgeI site found at 242
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1300